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ProLine Nitrifying Bacteria can be used to rapidly seed biological filters and correct ammonia and nitrite imbalances. Treatment rates are 4 ounces per 10 gallons for freshwater systems (use freshwater for salinity of 7 ppt or lower) and 8 ounces per 10 gallons for saltwater systems. For garden pond applications (fewer than 1,000 gallons), add four ounces per 20 gallons of water. For lightly loaded ponds or lakes, add two gallons per surface acre of water. For older or heavily loaded lakes, call a Pentair AES technician.